Show more...
Abstract:
Summary:
The data was collected during the Nansen Legacy Joint Cruise 3 from 19 February – 11 March 2022 on the research vessel RV Kronprins Haakon (cruise number 2022702), along a transect in the northern Barents Sea from 76° N to 82° N. The dataset contains abundance of pelagic marine protists, including phytoplankton (autotrophic) and protozooplankton (heterotrophic). Protists were identified and counted with light microscopy using the Utermöhl method and the results are given as cells per liter (cells/L) called organismQuantity.
Sampling method:
The samples were collected with Niskin bottles attached to a CTD rosette at the following depths: 0, 15, 30, 60, 90 m, and deep chlorophyll max (DCM) if it differed from the existing depths. The samples were preserved using an aldehyde mixture of glutaraldehyde and hexamethylenetetramine-buffered formalin at final concentrations of 0.1% and 1% respectively.
Analyse method:
All samples have been analysed at Institute of Oceanology of the Polish Academy of Sciences (IOPAN). The organisms were identified and counted under an inverted microscope according to the Utermöhl method.
Header name index - events
- expedition: cruise number for R/V Kronprins Haakon
- eventID: UUID for the sample
- parentID: UUID for the gear deployment (each Niskin has a unique parentID)
- eventDate: the date-time when an event occurred, using ISO 8601-1:2019 format (2020-07-27T07:16:03.44Z).
- fieldNumber: human-readable sample ID (e.g. PHT-001)
- locationID: station name
- decimalLongitude: geographic latitude (in decimal degrees, using the spatial reference system given in geodetic datum)
- decimalLatitude: geographic longitude (in decimal degrees, using the spatial reference system given in geodeticDatum)
- bottomDepthInMeters: bottom depth in meters
- eventRemarks: comments or remarks about the event (free text field)
- gearType: the gear used to take the sample e.g. Niskin bottle
- samplingDepthInMeters: depth sampled
- sampleType: description of the sample type according to a standard list
- recordedBy: name of the person who took the samples
- principalInvestigatorName: name of the person in charge of the sample collection
- principalInvestigatorEmail: email address of the person in charge of the sample collection
- principalInvestigatorInstitution: affiliated institution of the person in charge of the sample collection
Header name index - occurrence
- expedition: cruise number for R/V Kronprins Haakon
- eventID: UUID for the sample. Link the occurrence to the the event file
- phylum: a principal taxonomic category that ranks below kingdom and above class
- class: a taxonomical category that ranks below the phylum and above the order
- order: a taxonomical category that ranks below the class and above the family
- family: a taxonomical category that ranks below the order and above the genus
- genus: a taxonomical category that ranks below the genus and above the species
- taxonKey-LSID: ID for species used in Worms
- scientificName: full scientific name of the identified organism at the lowest taxonomic level that can be ascertained. The scientificName should be selected from a drop-down menu linked to the list in taxonomy sheet. (e.g Thalassiosira hyalina).
- identificationQualifier: A standard term (sp., spp., and indet.) to express uncertainty in identification.
- lifeStage: the life stage (e.g. resting spore) of the organism.
- sizeGroupOperator: describes if the size group is less than or greater than a value (It = less than, gte = greater or equal to)
- sizeGroup: the size group in µm.
- organismRemark: indicates e.g. varieties, colony type
- identificationRemarks: a free text field for adding information relevant to the analysis
- identifiedBy: person who did the lab-analyse
- fieldsInCount: Number of fields counted in the microscope
- magnificationMicroscope: The magnification setting used during analysis. Selected from a drop-down menu linked to vocab-sheet
- maxFields: Number of fields in the entire sedimentation chamber (Related to magnification used)
- takenVolumeML: The volume taken for sedimentation in the Utermöhl chamber (the sub-sample taken for analysis)
- identifiedBy: Drop-down menu linked to list in people-sheet
- dateIdentified: Date for the analysis
- sampleSizeValue=(fieldsInCount/maxFields)*(takenVolumeML/convertionMLtoL)*dilutionFactorFormaldehyde), dilutionFactorFormaldehyde = 0.95
- sampleSizeUnit: liter (l)
- organismQuantity: the quantity of the organism per volume water in the environment (organismQuantity = individualCount/sampleSizeValue)
- organismQuantityType: cells/l
Funding:
The Nansen Legacy is funded by the Research Council of Norway and the Norwegian Ministry of Education and Research. They provide 50% of the budget while the participating institutions contribute 50% in-kind. The total budget for the Nansen Legacy project is 740 mill. NOK.