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Abstract:
The data has been collected during the Nansen Legacy Seasonal Study Q2 from 27 April - 20 May 2021 on research vessel Kronprins Haakon (cruise number 2021704), along a transect in the northern Barents Sea from 76N to 82N. The dataset contains abundance of ice algae marine protists, including ice algae (autotrophic) and protozoa (heterotrophic). Protists were identified and counted with light microscopy using the Utermöhl method and the result are given as cells per liter (cells/L) called organismQuantity-
Quality
Sampling method:
This dataset exist of two occurence files, ice core samples & slurp gun samples. Ice core samples: The samples were collected with Kovacs ice corer 9 cm diameter (Kovacs Enterprise). The samples were collected from the bottom part of the ice core at the following dept layers: 0-3 cm, 3-10 cm, 10-20 cm & 20-30 cm. The ice cores were transferred to a clean bucket and 100mL filtered sea water were added per 1 cm of sea ice and melted at 4°C. When the ice core was melted, 95 mL of the sample was transferred into 100 ml brown glass bottle. The samples were preserved using an aldehyde mixture of glutaraldehyde and hexamethylenetetramine-buffered formalin at final concentrations of 0.1% and 1% respectively. Slurp gun samples: The samples were collected by divers using a slurp gun to suck up ice algae right underneath the sea ice over a swimming distance of 20 m.
Analyse method:
All samples have been analysed at Institute of Oceanology of the Polish Academy of Sciences (IOPAN). The organisms were identified and counted under an inverted microscope according to the Utermöhl method.
Header name index - events
- expedition: cruise number for R/V Kronprins Haakon
- eventID: UUID for the sample
- parentID: UUID for the gear deployment (each ice core has a unique parentID)
- eventDate: the date-time when an event occurred, using ISO 8601-1:2019 format (2020-07-27T07:16:03.446Z).
- fieldNumber: human-readable sample ID (e.g. IAT-001)
- locationID: station name
- decimalLongitude: geographic latitude (in decimal degrees, using the spatial reference system given in geodetic datum)
- decimalLatitude: geographic longitude (in decimal degrees, using the spatial reference system given in geodeticDatum)
- maximumDepthInCentimeters: bottom depth of the core section in cm
- minimumDepthInCentimeters: upper depth of the core section in cm
- eventRemarks: comments or remarks about the event (free text field)
- gearType: the gear used to take the sample e.g. Ice corer 9 cm
- samplingDepthInMeters: depth sampled
- sampleType: description of the sample type according to a standard list
- recordedBy: name of the person who took the samples
- principalInvestigatorName: name of the person in charge of the sample collection
- principalInvestigatorEmail: email address of the person in charge of the sample collection
- principalInvestigatorInstitution: affiliated institution of the person in charge of the sample collection
Header name index - occurrence
- scientificName: full scientific name of the identified organism at the lowest taxonomic level that can be ascertained. The scientificName should be selected from a drop-down menu linked to the list in taxonomy sheet. (e.g Nitzschia frigida).
- identificationQualifier: A standard term (sp., spp., and indet.) to express the determiner’s doubts about the Identification.
- lifeStage: the life stage (e.g. resting spore) of the organism
- sizeGroupOperator: describes if the size group is less than or greater than a value (It = less than, gte = greater or equal to)
- sizeGroup: the size group in µm.
- organismRemark: indicates e.g. varieties, colony type
- identificationRemarks: a free text field for adding information relevant to the analysis
- identifiedBy: person who did the lab-analyse
- identifiedBy: Drop-down menu linked to list in people-sheet
- dateIdentified: Date for the analysis
- fieldsInCount: Number of fields counted in the microscope
- magnificationMicroscope: The magnification setting used during analysis. Selected from a drop-down menu linked to vocab-sheet
- maxFields: Number of fields in the entire sedimentation chamber (Related to magnification used)
- takenVolumeML: The volume taken for sedimentation in the Utermöhl chamber (the sub-sample taken for analysis)
- totalMeltedVolumeL: The total melted volume in L recorded during sampling.
- addedFSWvolumeL: Volume in L of filtered sea water added to the sample during melting.
- initialVolumeL: The total volume in L of the melted core, measured during sampling. If it wasn’t measured one can use the theoretical calculated core volume based on diameter of the core. initialVolumeL=(totalMeltedVolumeL-addedFSWvolumeL)) or teoreticalCoreVolumeL = coreAreM*(maxDepthCM-minDepthCM)
- sampleSizeValue=((fieldsInCount/maxFields)(takenVolumeML/conversionMLtoL))(dilutionFactorFormaldehyde*dilutionFactorFSW)), dilutionFactorFormaldehyde = 0.95, dilutionFactorFSW=
- sampleSizeUnit: liter (l)
- organismQuantity: the quantity of the organism per volume water in the environment (organismQuantity = individualCount/sampleSizeValue)
- organismQuantityType: cells/l
- cellsPerM2: The quantity (number of cells) of the organism per area (m2). cellsPerM2 = ((individualCount/(sampleSizeValue/initialVolumeL))/coreAreaM
Funding:
The Nansen Legacy is funded by the Research Council of Norway and the Norwegian Ministry of Education and Research. They provide 50% of the budget while the participating institutions contribute 50% in-kind. The total budget for the Nansen Legacy project is 740 mill. NOK.